DNA

Part:BBa_K4118000:Design

Designed by: Eleftherios Bochalis   Group: iGEM22_Patras_Medicine   (2022-10-09)


RCA Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa K41180000 is designed by Patras Medicine and their design is evaluated with the use of ldn_generator.py

Source

The RCA domain originates from a phosphorylated DNA template sequence and a primer complementary to the two ends of the template in order to bring them closer, creating a padlock DNA template.

References

(1). Zhao, W., Cui, C. H., Bose, S., Guo, D., Shen, C., Wong, W. P., Halvorsen, K., Farokhzad, O. C., Teo, G. S. L., Phillips, J. A., Dorfman, D. M., Karnik, R., & Karp, J. M. (2012, November 12). Bioinspired multivalent DNA network for capture and release of cells. Proceedings of the National Academy of Sciences, 109(48), 19626–19631. https://doi.org/10.1073/pnas.1211234109